maximum_oligo_size = 70
minimum_oligo_size = 50
maximum_match_len = 15
minimum_match_len = 10
maximum_gc_content = 70
minimum_gc_content = 30
candidates_per_gene = 5
probes_per_gene = 1
minimum_similarity = 75
minimum_temp_separation = 20
DNA_concentration = 1
Salt_concentration = 75
single_strand_only = 0
44729 target sequences.
44729 total sequences.
26743158 total DNA bases.
4032 is the longest sequence length.
11021 sequences were dead due to short overlaps with others.
3572 sequences were dead due to long overlaps with others.
2651 sequences were dead with no good probe candidates.
70791 initial best probe candidates detected.
8669 sequences without probe due to temperature conflicts.
18816 total oligo probes designed for 18816 sequences.
63.64 C is the optimal estimated experiment temperature.
total time elapsed 2006 seconds