maximum_oligo_size = 70
minimum_oligo_size = 50
maximum_match_len = 16
minimum_match_len = 10
maximum_gc_content = 70
minimum_gc_content = 30
candidates_per_gene = 5
probes_per_gene = 1
minimum_similarity = 75
minimum_temp_separation = 10
DNA_concentration = 1
Salt_concentration = 75
single_strand_only = 0
44729 target sequences.
44729 total sequences.
26743158 total DNA bases.
4032 is the longest sequence length.
3460 sequences were dead due to short overlaps with others.
2125 sequences were dead due to long overlaps with others.
1048 sequences were dead with no good probe candidates.
129952 initial best probe candidates detected.
3074 sequences without probe due to temperature conflicts.
35022 total oligo probes designed for 35022 sequences.
68.41 C is the optimal estimated experiment temperature.
total time elapsed 10526 seconds