maximum_oligo_size = 70
minimum_oligo_size = 50
maximum_match_len = 16
minimum_match_len = 10
maximum_gc_content = 70
minimum_gc_content = 30
candidates_per_gene = 5
probes_per_gene = 1
minimum_similarity = 75
minimum_temp_separation = 20
DNA_concentration = 1
Salt_concentration = 75
single_strand_only = 0
44729 target sequences.
44729 total sequences.
26743158 total DNA bases.
4032 is the longest sequence length.
3460 sequences were dead due to short overlaps with others.
2125 sequences were dead due to long overlaps with others.
3497 sequences were dead with no good probe candidates.
114405 initial best probe candidates detected.
11190 sequences without probe due to temperature conflicts.
24457 total oligo probes designed for 24457 sequences.
65.39 C is the optimal estimated experiment temperature.
total time elapsed 7768 seconds