maximum_oligo_size = 70
minimum_oligo_size = 50
maximum_match_len = 15
minimum_match_len = 10
maximum_gc_content = 70
minimum_gc_content = 30
candidates_per_gene = 5
probes_per_gene = 1
minimum_similarity = 75
minimum_temp_separation = 20
DNA_concentration = 1
Salt_concentration = 75
single_strand_only = 0
25392 target sequences.
25392 total sequences.
52701574 total DNA bases.
16380 is the longest sequence length.
2594 sequences were dead due to short overlaps with others.
5184 sequences were dead due to long overlaps with others.
909 sequences were dead with no good probe candidates.
49130 initial best probe candidates detected.
5666 sequences without probe due to temperature conflicts.
11039 total oligo probes designed for 11039 sequences.
63.95 C is the optimal estimated experiment temperature.
total time elapsed 4225 seconds