maximum_oligo_size = 70
minimum_oligo_size = 50
maximum_match_len = 16
minimum_match_len = 10
maximum_gc_content = 70
minimum_gc_content = 30
candidates_per_gene = 5
probes_per_gene = 1
minimum_similarity = 75
minimum_temp_separation = 10
DNA_concentration = 1
Salt_concentration = 75
single_strand_only = 0
25392 target sequences.
25392 total sequences.
52701574 total DNA bases.
16380 is the longest sequence length.
160 sequences were dead due to short overlaps with others.
3008 sequences were dead due to long overlaps with others.
292 sequences were dead with no good probe candidates.
99587 initial best probe candidates detected.
469 sequences without probe due to temperature conflicts.
21463 total oligo probes designed for 21463 sequences.
65.28 C is the optimal estimated experiment temperature.
total time elapsed 56292 seconds