;; This buffer is for notes you don't want to save, and for Lisp evaluation.
;; If you want to create a file, visit that file with C-x C-f,
;; then enter the maximum_oligo_size = 70
minimum_oligo_size = 50
maximum_match_len = 16
minimum_match_len = 10
maximum_gc_content = 70
minimum_gc_content = 30
candidates_per_gene = 5
probes_per_gene = 1
minimum_similarity = 75
minimum_temp_separation = 20
DNA_concentration = 1
Salt_concentration = 75
single_strand_only = 0
25392 target sequences.
25392 total sequences.
52701574 total DNA bases.
16380 is the longest sequence length.
160 sequences were dead due to short overlaps with others.
3008 sequences were dead due to long overlaps with others.
844 sequences were dead with no good probe candidates.
96332 initial best probe candidates detected.
2633 sequences without probe due to temperature conflicts.
18747 total oligo probes designed for 18747 sequences.
64.09 C is the optimal estimated experiment temperature.
total time elapsed 57145 seconds
text in that file's own buffer.