Picky parameter settings for this computation: maximum_oligo_size = 70 minimum_oligo_size = 50 maximum_match_len = 16 minimum_match_len = 10 maximum_gc_content = 70 minimum_gc_content = 30 candidates_per_gene = 5 probes_per_gene = 1 minimum_similarity = 75 minimum_temp_separation = 10 DNA_concentration = 1 Salt_concentration = 75 single_strand_only = 0 Job started on Fri 27 May 2005 08:15:47 PM CDT Step 1 of 6: Constructing the suffix array... 30935 target sequences. 30935 total sequences. 34783951 total DNA bases. 16380 is the longest sequence length. Step 2 of 6: Computing invert and lcp arrays... 16380 is the longest common overlap region length among sequences. 115.38 is the average common overlap region length among sequences. Step 3 of 6: Generating unique probe candidates... 4 CPU(s) used in this computation; step 3 is slower, be patient.... 711 sequences have no unique probe due to short overlaps with others. 7288 sequences have no unique probe due to long overlaps with others. 176 sequences have no good probe candidates due to temperature limit. 92974 initial unique probe candidates detected. Step 4 of 6: Identifying long common overlap regions... 14681 potentially sharable long common overlap regions identified. 7588 sequences hosting long common overlap regions identified. Step 5 of 6: Generating shared probe candidates... 4 CPU(s) used in this computation; step 5 is also slow, be patient... 315 host sequences were not useful due to short overlaps. 1475 host sequences were taken by the other host sequences. 846 host sequences produce no probes due to temperature limit. 17784 additional sharable probe candidates detected. Step 6 of 6: Determining the optimal oligo set... 832 sequences/regions without probe at 62.56 C, 216 below, 616 above. 862 sequences/regions without probe at 63.01 C, 308 below, 554 above. 928 sequences/regions without probe at 63.53 C, 436 below, 492 above. 976 sequences/regions without probe at 63.94 C, 524 below, 452 above. 832 sequences/regions without probe at 10.00 separation temperature. 22239 unique oligo probes designed for 22239 sequences. 2621 shared oligo probes designed for 3137 additional sequences. 11 maximum number of sequences sharing the same oligo probe. 2.27 average number of sequences sharing the same oligo probe. 57.56 C is the maximum estimated probe-nontarget temperature. 67.57 C is the minimum estimated probe-target temperature. 62.56 C is the optimal estimated experiment temperature. 24860 total oligo probes designed for 25376 sequences. Job completed on Fri 27 May 2005 10:20:46 PM CDT Total time elapsed 02h:04m:59s.