Picky parameter settings for this computation: maximum_oligo_size = 70 minimum_oligo_size = 50 maximum_match_len = 15 minimum_match_len = 10 maximum_gc_content = 70 minimum_gc_content = 30 candidates_per_gene = 5 probes_per_gene = 1 minimum_similarity = 75 minimum_temp_separation = 10 DNA_concentration = 1 Salt_concentration = 75 single_strand_only = 0 Job started on Sun 15 May 2005 08:21:05 PM CDT Step 1 of 6: Constructing the suffix array... 18962 target sequences. 18962 total sequences. 32217720 total DNA bases. 16380 is the longest sequence length. Step 2 of 6: Computing invert and lcp arrays... 16380 is the longest common overlap region length among sequences. 485.55 is the average common overlap region length among sequences. Step 3 of 6: Generating unique probe candidates... 4 CPU(s) used in this computation; step 3 is slower, be patient.... 337 sequences have no unique probe due to short overlaps with others. 7096 sequences have no unique probe due to long overlaps with others. 7 sequences have no good probe candidates due to temperature limit. 45256 initial unique probe candidates detected. Step 4 of 6: Identifying long common overlap regions... 6465 potentially sharable long common overlap regions identified. 4186 sequences hosting long common overlap regions identified. Step 5 of 6: Generating shared probe candidates... 4 CPU(s) used in this computation; step 5 is also slow, be patient... 105 host sequences were not useful due to short overlaps. 491 host sequences were taken by the other host sequences. 45 host sequences produce no probes due to temperature limit. 15774 additional sharable probe candidates detected. Step 6 of 6: Determining the optimal oligo set... 501 sequences/regions without probe at 64.51 C, 74 below, 427 above. 367 sequences/regions without probe at 66.49 C, 163 below, 204 above. 361 sequences/regions without probe at 66.95 C, 196 below, 165 above. 361 sequences/regions without probe at 10.00 separation temperature. 11299 unique oligo probes designed for 11299 sequences. 3296 shared oligo probes designed for 6895 additional sequences. 26 maximum number of sequences sharing the same oligo probe. 2.81 average number of sequences sharing the same oligo probe. 61.95 C is the maximum estimated probe-nontarget temperature. 71.95 C is the minimum estimated probe-target temperature. 66.95 C is the optimal estimated experiment temperature. 14595 total oligo probes designed for 18194 sequences. Job completed on Sun 15 May 2005 08:52:21 PM CDT Total time elapsed 00h:31m:16s.