Picky parameter settings for this computation:
maximum_oligo_size = 70
minimum_oligo_size = 50
maximum_match_len = 17
minimum_match_len = 10
maximum_gc_content = 70
minimum_gc_content = 30
candidates_per_gene = 5
probes_per_gene = 1
minimum_similarity = 75
minimum_temp_separation = 10
DNA_concentration = 1
Salt_concentration = 75
single_strand_only = 0

Job started on Sat 14 May 2005 05:07:52 PM CDT
Step 1 of 6: Constructing the suffix array...
18962 target sequences.
18962 total sequences.
32217720 total DNA bases.
16380 is the longest sequence length.
Step 2 of 6: Computing invert and lcp arrays...
16380 is the longest common overlap region length among sequences.
485.55 is the average common overlap region length among sequences.
Step 3 of 6: Generating unique probe candidates...
4 CPU(s) used in this computation; step 3 is slower, be patient....
3 sequences have no unique probe due to short overlaps with others.
6466 sequences have no unique probe due to long overlaps with others.
14 sequences have no good probe candidates due to temperature limit.
55963 initial unique probe candidates detected.
Step 4 of 6: Identifying long common overlap regions...
6530 potentially sharable long common overlap regions identified.
4194 sequences hosting long common overlap regions identified.
Step 5 of 6: Generating shared probe candidates...
4 CPU(s) used in this computation; step 5 is also slow, be patient...
13 host sequences were not useful due to short overlaps.
202 host sequences were taken by the other host sequences.
260 host sequences produce no probes due to temperature limit.
19473 additional sharable probe candidates detected.
Step 6 of 6: Determining the optimal oligo set...
332 sequences/regions without probe at 65.07 C, 74 below, 258 above.
289 sequences/regions without probe at 66.71 C, 148 below, 141 above.
289 sequences/regions without probe at 10.00 separation temperature.
12310 unique oligo probes designed for 12310 sequences.
3376 shared oligo probes designed for 6527 additional sequences.
26 maximum number of sequences sharing the same oligo probe.
2.78 average number of sequences sharing the same oligo probe.
61.70 C is the maximum estimated probe-nontarget temperature.
71.71 C is the minimum estimated probe-target temperature.
66.71 C is the optimal estimated experiment temperature.
15686 total oligo probes designed for 18837 sequences.
Job completed on Sat 14 May 2005 07:26:53 PM CDT
Total time elapsed 02h:19m:01s.