Picky parameter settings for this computation:
maximum_oligo_size = 70
minimum_oligo_size = 50
maximum_match_len = 16
minimum_match_len = 10
maximum_gc_content = 70
minimum_gc_content = 30
candidates_per_gene = 5
probes_per_gene = 1
minimum_similarity = 75
minimum_temp_separation = 20
DNA_concentration = 1
Salt_concentration = 75
single_strand_only = 0

Job started on Tue 24 May 2005 02:48:22 PM CDT
Step 1 of 6: Constructing the suffix array...
5317 target sequences.
5317 total sequences.
4848788 total DNA bases.
15567 is the longest sequence length.
Step 2 of 6: Computing invert and lcp arrays...
3018 is the longest common overlap region length among sequences.
28.70 is the average common overlap region length among sequences.
Step 3 of 6: Generating unique probe candidates...
2 CPU(s) used in this computation; step 3 is slower, be patient....
12 sequences have no unique probe due to short overlaps with others.
561 sequences have no unique probe due to long overlaps with others.
70 sequences have no good probe candidates due to temperature limit.
21552 initial unique probe candidates detected.
Step 4 of 6: Identifying long common overlap regions...
1100 potentially sharable long common overlap regions identified.
474 sequences hosting long common overlap regions identified.
Step 5 of 6: Generating shared probe candidates...
2 CPU(s) used in this computation; step 5 is also slow, be patient...
23 host sequences were not useful due to short overlaps.
97 host sequences were taken by the other host sequences.
122 host sequences produce no probes due to temperature limit.
680 additional sharable probe candidates detected.
Step 6 of 6: Determining the optimal oligo set...
234 sequences/regions without probe at 62.39 C, 75 below, 159 above.
252 sequences/regions without probe at 63.43 C, 124 below, 128 above.
245 sequences/regions without probe at 63.79 C, 132 below, 113 above.
234 sequences/regions without probe at 20.00 separation temperature.
4524 unique oligo probes designed for 4524 sequences.
155 shared oligo probes designed for 327 additional sequences.
10 maximum number of sequences sharing the same oligo probe.
2.34 average number of sequences sharing the same oligo probe.
52.39 C is the maximum estimated probe-nontarget temperature.
72.41 C is the minimum estimated probe-target temperature.
62.39 C is the optimal estimated experiment temperature.
4679 total oligo probes designed for 4851 sequences.
Job completed on Tue 24 May 2005 03:03:05 PM CDT
Total time elapsed 00h:14m:43s.