Picky parameter settings for this computation:
maximum_oligo_size = 70
minimum_oligo_size = 50
maximum_match_len = 16
minimum_match_len = 10
maximum_gc_content = 70
minimum_gc_content = 30
candidates_per_gene = 5
probes_per_gene = 1
minimum_similarity = 75
minimum_temp_separation = 10
DNA_concentration = 1
Salt_concentration = 75
single_strand_only = 0

Job started on Wed 25 May 2005 01:01:19 PM CDT
Step 1 of 6: Constructing the suffix array...
11324 target sequences.
11324 total sequences.
6022273 total DNA bases.
5426 is the longest sequence length.
Step 2 of 6: Computing invert and lcp arrays...
982 is the longest common overlap region length among sequences.
13.96 is the average common overlap region length among sequences.
Step 3 of 6: Generating unique probe candidates...
4 CPU(s) used in this computation; step 3 is slower, be patient....
159 sequences have no unique probe due to short overlaps with others.
249 sequences have no unique probe due to long overlaps with others.
18 sequences have no good probe candidates due to temperature limit.
45384 initial unique probe candidates detected.
Step 4 of 6: Identifying long common overlap regions...
897 potentially sharable long common overlap regions identified.
620 sequences hosting long common overlap regions identified.
Step 5 of 6: Generating shared probe candidates...
4 CPU(s) used in this computation; step 5 is also slow, be patient...
54 host sequences were not useful due to short overlaps.
41 host sequences were taken by the other host sequences.
228 host sequences produce no probes due to temperature limit.
592 additional sharable probe candidates detected.
Step 6 of 6: Determining the optimal oligo set...
53 sequences/regions without probe at 59.60 C, 1 below, 52 above.
53 sequences/regions without probe at 61.15 C, 19 below, 34 above.
77 sequences/regions without probe at 62.05 C, 53 below, 24 above.
53 sequences/regions without probe at 10.00 separation temperature.
10852 unique oligo probes designed for 10852 sequences.
115 shared oligo probes designed for 143 additional sequences.
6 maximum number of sequences sharing the same oligo probe.
2.10 average number of sequences sharing the same oligo probe.
54.60 C is the maximum estimated probe-nontarget temperature.
64.62 C is the minimum estimated probe-target temperature.
59.60 C is the optimal estimated experiment temperature.
10967 total oligo probes designed for 10995 sequences.
Job completed on Wed 25 May 2005 01:14:08 PM CDT
Total time elapsed 00h:12m:49s.