Picky parameter settings for this computation: maximum_oligo_size = 70 minimum_oligo_size = 50 maximum_match_len = 15 minimum_match_len = 10 maximum_gc_content = 70 minimum_gc_content = 30 candidates_per_gene = 5 probes_per_gene = 1 minimum_similarity = 75 minimum_temp_separation = 10 DNA_concentration = 1 Salt_concentration = 75 single_strand_only = 0 Job started on Wed 11 May 2005 01:24:55 PM CDT Step 1 of 6: Constructing the suffix array... 26236 target sequences. 26236 total sequences. 32759147 total DNA bases. 15717 is the longest sequence length. Step 2 of 6: Computing invert and lcp arrays... 9948 is the longest common overlap region length among sequences. 92.75 is the average common overlap region length among sequences. Step 3 of 6: Generating unique probe candidates... 2 CPU(s) used in this computation; step 3 is slower, be patient.... 304 sequences have no unique probe due to short overlaps with others. 10027 sequences have no unique probe due to long overlaps with others. 31 sequences have no good probe candidates due to temperature limit. 56130 initial unique probe candidates detected. Step 4 of 6: Identifying long common overlap regions... 29239 potentially sharable long common overlap regions identified. 11246 sequences hosting long common overlap regions identified. Step 5 of 6: Generating shared probe candidates... 2 CPU(s) used in this computation; step 5 is also slow, be patient... 296 host sequences were not useful due to short overlaps. 3636 host sequences were taken by the other host sequences. 396 host sequences produce no probes due to temperature limit. 17845 additional sharable probe candidates detected. Step 6 of 6: Determining the optimal oligo set... 1833 sequences/regions without probe at 62.98 C, 219 below, 1614 above. 1728 sequences/regions without probe at 65.11 C, 754 below, 974 above. 1953 sequences/regions without probe at 65.99 C, 1178 below, 775 above. 1728 sequences/regions without probe at 10.00 separation temperature. 15260 unique oligo probes designed for 15260 sequences. 5011 shared oligo probes designed for 8099 additional sequences. 20 maximum number of sequences sharing the same oligo probe. 2.50 average number of sequences sharing the same oligo probe. 60.10 C is the maximum estimated probe-nontarget temperature. 70.11 C is the minimum estimated probe-target temperature. 65.11 C is the optimal estimated experiment temperature. 20271 total oligo probes designed for 23359 sequences. Job completed on Wed 11 May 2005 02:12:43 PM CDT Total time elapsed 00h:47m:48s.