Picky parameter settings for this computation:
maximum_oligo_size = 70
minimum_oligo_size = 50
maximum_match_len = 17
minimum_match_len = 10
maximum_gc_content = 70
minimum_gc_content = 30
candidates_per_gene = 5
probes_per_gene = 1
minimum_similarity = 75
minimum_temp_separation = 20
DNA_concentration = 1
Salt_concentration = 75
single_strand_only = 0

Job started on Thu 12 May 2005 09:39:05 AM CDT
Step 1 of 6: Constructing the suffix array...
26236 target sequences.
26236 total sequences.
32759147 total DNA bases.
15717 is the longest sequence length.
Step 2 of 6: Computing invert and lcp arrays...
9948 is the longest common overlap region length among sequences.
92.75 is the average common overlap region length among sequences.
Step 3 of 6: Generating unique probe candidates...
2 CPU(s) used in this computation; step 3 is slower, be patient....
33 sequences have no unique probe due to short overlaps with others.
8006 sequences have no unique probe due to long overlaps with others.
433 sequences have no good probe candidates due to temperature limit.
79183 initial unique probe candidates detected.
Step 4 of 6: Identifying long common overlap regions...
29564 potentially sharable long common overlap regions identified.
11277 sequences hosting long common overlap regions identified.
Step 5 of 6: Generating shared probe candidates...
2 CPU(s) used in this computation; step 5 is also slow, be patient...
49 host sequences were not useful due to short overlaps.
1066 host sequences were taken by the other host sequences.
2192 host sequences produce no probes due to temperature limit.
30596 additional sharable probe candidates detected.
Step 6 of 6: Determining the optimal oligo set...
6421 sequences/regions without probe at 63.24 C, 2033 below, 4388 above.
6404 sequences/regions without probe at 63.38 C, 2133 below, 4271 above.
6404 sequences/regions without probe at 63.68 C, 2374 below, 4030 above.
6459 sequences/regions without probe at 63.85 C, 2523 below, 3936 above.
6483 sequences/regions without probe at 64.00 C, 2669 below, 3814 above.
6535 sequences/regions without probe at 64.15 C, 2807 below, 3728 above.
6739 sequences/regions without probe at 64.59 C, 3269 below, 3470 above.
6878 sequences/regions without probe at 64.87 C, 3573 below, 3305 above.
6878 sequences/regions without probe at 64.85 C, 3559 below, 3319 above.
6782 sequences/regions without probe at 64.68 C, 3373 below, 3409 above.
6404 sequences/regions without probe at 20.00 separation temperature.
15841 unique oligo probes designed for 15841 sequences.
4536 shared oligo probes designed for 7205 additional sequences.
20 maximum number of sequences sharing the same oligo probe.
2.49 average number of sequences sharing the same oligo probe.
53.38 C is the maximum estimated probe-nontarget temperature.
73.38 C is the minimum estimated probe-target temperature.
63.38 C is the optimal estimated experiment temperature.
20377 total oligo probes designed for 23046 sequences.
Job completed on Thu 12 May 2005 05:02:22 PM CDT
Total time elapsed 07h:23m:17s.