Picky parameter settings for this computation:
maximum_oligo_size = 70
minimum_oligo_size = 50
maximum_match_len = 17
minimum_match_len = 10
maximum_gc_content = 70
minimum_gc_content = 30
candidates_per_gene = 5
probes_per_gene = 1
minimum_similarity = 75
minimum_temp_separation = 20
DNA_concentration = 1
Salt_concentration = 75
single_strand_only = 0

Job started on Tue 10 May 2005 11:54:34 AM CDT
Step 1 of 6: Constructing the suffix array...
28205 target sequences.
28205 total sequences.
72748721 total DNA bases.
16380 is the longest sequence length.
Step 2 of 6: Computing invert and lcp arrays...
16179 is the longest common overlap region length among sequences.
292.92 is the average common overlap region length among sequences.
Step 3 of 6: Generating unique probe candidates...
4 CPU(s) used in this computation; step 3 is slower, be patient....
14 sequences have no unique probe due to short overlaps with others.
5338 sequences have no unique probe due to long overlaps with others.
892 sequences have no good probe candidates due to temperature limit.
98455 initial unique probe candidates detected.
Step 4 of 6: Identifying long common overlap regions...
22730 potentially sharable long common overlap regions identified.
8380 sequences hosting long common overlap regions identified.
Step 5 of 6: Generating shared probe candidates...
4 CPU(s) used in this computation; step 5 is also slow, be patient...
151 host sequences were not useful due to short overlaps.
1648 host sequences were taken by the other host sequences.
1286 host sequences produce no probes due to temperature limit.
27352 additional sharable probe candidates detected.
Step 6 of 6: Determining the optimal oligo set...
6838 sequences/regions without probe at 64.19 C, 2081 below, 4757 above.
6832 sequences/regions without probe at 64.28 C, 2169 below, 4663 above.
6834 sequences/regions without probe at 64.38 C, 2240 below, 4594 above.
6808 sequences/regions without probe at 64.55 C, 2343 below, 4465 above.
6812 sequences/regions without probe at 64.65 C, 2432 below, 4380 above.
6844 sequences/regions without probe at 64.94 C, 2693 below, 4151 above.
6846 sequences/regions without probe at 65.06 C, 2789 below, 4057 above.
6845 sequences/regions without probe at 65.28 C, 2991 below, 3854 above.
6909 sequences/regions without probe at 65.44 C, 3148 below, 3761 above.
6959 sequences/regions without probe at 65.51 C, 3252 below, 3707 above.
6971 sequences/regions without probe at 65.54 C, 3280 below, 3691 above.
7023 sequences/regions without probe at 65.61 C, 3371 below, 3652 above.
7024 sequences/regions without probe at 65.62 C, 3381 below, 3643 above.
7099 sequences/regions without probe at 65.77 C, 3561 below, 3538 above.
6808 sequences/regions without probe at 20.00 separation temperature.
18438 unique oligo probes designed for 18438 sequences.
3051 shared oligo probes designed for 5765 additional sequences.
24 maximum number of sequences sharing the same oligo probe.
2.67 average number of sequences sharing the same oligo probe.
54.54 C is the maximum estimated probe-nontarget temperature.
74.55 C is the minimum estimated probe-target temperature.
64.55 C is the optimal estimated experiment temperature.
21489 total oligo probes designed for 24203 sequences.
Job completed on Tue 10 May 2005 08:58:29 PM CDT
Total time elapsed 09h:03m:55s.