Picky parameter settings for this computation:
maximum_oligo_size = 70
minimum_oligo_size = 50
maximum_match_len = 15
minimum_match_len = 10
maximum_gc_content = 70
minimum_gc_content = 30
candidates_per_gene = 5
probes_per_gene = 1
minimum_similarity = 75
minimum_temp_separation = 10
DNA_concentration = 1
Salt_concentration = 75
single_strand_only = 0

Job started on Wed 18 May 2005 04:04:40 PM CDT
Step 1 of 6: Constructing the suffix array...
58579 target sequences.
58579 total sequences.
39022169 total DNA bases.
8251 is the longest sequence length.
Step 2 of 6: Computing invert and lcp arrays...
3266 is the longest common overlap region length among sequences.
24.32 is the average common overlap region length among sequences.
Step 3 of 6: Generating unique probe candidates...
2 CPU(s) used in this computation; step 3 is slower, be patient....
7422 sequences have no unique probe due to short overlaps with others.
13166 sequences have no unique probe due to long overlaps with others.
944 sequences have no good probe candidates due to temperature limit.
90762 initial unique probe candidates detected.
Step 4 of 6: Identifying long common overlap regions...
24250 potentially sharable long common overlap regions identified.
12914 sequences hosting long common overlap regions identified.
Step 5 of 6: Generating shared probe candidates...
2 CPU(s) used in this computation; step 5 is also slow, be patient...
3013 host sequences were not useful due to short overlaps.
6352 host sequences were taken by the other host sequences.
890 host sequences produce no probes due to temperature limit.
3765 additional sharable probe candidates detected.
Step 6 of 6: Determining the optimal oligo set...
4741 sequences/regions without probe at 63.65 C, 775 below, 3966 above.
4656 sequences/regions without probe at 64.02 C, 951 below, 3705 above.
4514 sequences/regions without probe at 64.50 C, 1209 below, 3305 above.
4507 sequences/regions without probe at 64.65 C, 1305 below, 3202 above.
4530 sequences/regions without probe at 65.19 C, 1680 below, 2850 above.
4525 sequences/regions without probe at 65.29 C, 1742 below, 2783 above.
4721 sequences/regions without probe at 65.86 C, 2284 below, 2437 above.
4839 sequences/regions without probe at 66.20 C, 2606 below, 2233 above.
4507 sequences/regions without probe at 10.00 separation temperature.
33027 unique oligo probes designed for 33027 sequences.
1696 shared oligo probes designed for 2262 additional sequences.
5 maximum number of sequences sharing the same oligo probe.
2.10 average number of sequences sharing the same oligo probe.
59.65 C is the maximum estimated probe-nontarget temperature.
69.66 C is the minimum estimated probe-target temperature.
64.65 C is the optimal estimated experiment temperature.
34723 total oligo probes designed for 35289 sequences.
Job completed on Wed 18 May 2005 04:52:52 PM CDT
Total time elapsed 00h:48m:12s.