Picky parameter settings for this computation:
maximum_oligo_size = 70
minimum_oligo_size = 50
maximum_match_len = 15
minimum_match_len = 10
maximum_gc_content = 70
minimum_gc_content = 30
candidates_per_gene = 5
probes_per_gene = 1
minimum_similarity = 75
minimum_temp_separation = 10
DNA_concentration = 1
Salt_concentration = 75
single_strand_only = 0

Job started on Thu 19 May 2005 10:30:01 AM CDT
Step 1 of 6: Constructing the suffix array...
35284 target sequences.
35284 total sequences.
68639601 total DNA bases.
16380 is the longest sequence length.
Step 2 of 6: Computing invert and lcp arrays...
16380 is the longest common overlap region length among sequences.
213.78 is the average common overlap region length among sequences.
Step 3 of 6: Generating unique probe candidates...
4 CPU(s) used in this computation; step 3 is slower, be patient....
2780 sequences have no unique probe due to short overlaps with others.
15666 sequences have no unique probe due to long overlaps with others.
105 sequences have no good probe candidates due to temperature limit.
46080 initial unique probe candidates detected.
Step 4 of 6: Identifying long common overlap regions...
28322 potentially sharable long common overlap regions identified.
12564 sequences hosting long common overlap regions identified.
Step 5 of 6: Generating shared probe candidates...
4 CPU(s) used in this computation; step 5 is also slow, be patient...
569 host sequences were not useful due to short overlaps.
7341 host sequences were taken by the other host sequences.
149 host sequences produce no probes due to temperature limit.
10949 additional sharable probe candidates detected.
Step 6 of 6: Determining the optimal oligo set...
2086 sequences/regions without probe at 64.18 C, 412 below, 1674 above.
2069 sequences/regions without probe at 64.44 C, 474 below, 1595 above.
2019 sequences/regions without probe at 64.68 C, 530 below, 1489 above.
1957 sequences/regions without probe at 65.55 C, 805 below, 1152 above.
2006 sequences/regions without probe at 65.96 C, 970 below, 1036 above.
2105 sequences/regions without probe at 66.57 C, 1247 below, 858 above.
2036 sequences/regions without probe at 66.08 C, 1032 below, 1004 above.
1957 sequences/regions without probe at 10.00 separation temperature.
15365 unique oligo probes designed for 15365 sequences.
3501 shared oligo probes designed for 5936 additional sequences.
13 maximum number of sequences sharing the same oligo probe.
2.36 average number of sequences sharing the same oligo probe.
60.55 C is the maximum estimated probe-nontarget temperature.
70.55 C is the minimum estimated probe-target temperature.
65.55 C is the optimal estimated experiment temperature.
18866 total oligo probes designed for 21301 sequences.
Job completed on Thu 19 May 2005 10:53:36 AM CDT
Total time elapsed 00h:23m:35s.