Picky parameter settings for this computation:
maximum_oligo_size = 70
minimum_oligo_size = 50
maximum_match_len = 15
minimum_match_len = 10
maximum_gc_content = 70
minimum_gc_content = 30
candidates_per_gene = 5
probes_per_gene = 1
minimum_similarity = 75
minimum_temp_separation = 20
DNA_concentration = 1
Salt_concentration = 75
single_strand_only = 0

Job started on Thu 19 May 2005 10:02:34 AM CDT
Step 1 of 6: Constructing the suffix array...
35284 target sequences.
35284 total sequences.
68639601 total DNA bases.
16380 is the longest sequence length.
Step 2 of 6: Computing invert and lcp arrays...
16380 is the longest common overlap region length among sequences.
213.78 is the average common overlap region length among sequences.
Step 3 of 6: Generating unique probe candidates...
4 CPU(s) used in this computation; step 3 is slower, be patient....
2780 sequences have no unique probe due to short overlaps with others.
15666 sequences have no unique probe due to long overlaps with others.
1156 sequences have no good probe candidates due to temperature limit.
40323 initial unique probe candidates detected.
Step 4 of 6: Identifying long common overlap regions...
28322 potentially sharable long common overlap regions identified.
12564 sequences hosting long common overlap regions identified.
Step 5 of 6: Generating shared probe candidates...
4 CPU(s) used in this computation; step 5 is also slow, be patient...
569 host sequences were not useful due to short overlaps.
7341 host sequences were taken by the other host sequences.
528 host sequences produce no probes due to temperature limit.
9336 additional sharable probe candidates detected.
Step 6 of 6: Determining the optimal oligo set...
8818 sequences/regions without probe at 63.89 C, 3744 below, 5074 above.
8818 sequences/regions without probe at 64.04 C, 3889 below, 4929 above.
8810 sequences/regions without probe at 64.13 C, 3975 below, 4835 above.
8857 sequences/regions without probe at 64.31 C, 4176 below, 4681 above.
8881 sequences/regions without probe at 64.43 C, 4293 below, 4588 above.
8908 sequences/regions without probe at 64.49 C, 4381 below, 4527 above.
8902 sequences/regions without probe at 64.57 C, 4462 below, 4440 above.
8810 sequences/regions without probe at 20.00 separation temperature.
9234 unique oligo probes designed for 9234 sequences.
2216 shared oligo probes designed for 4258 additional sequences.
11 maximum number of sequences sharing the same oligo probe.
2.33 average number of sequences sharing the same oligo probe.
54.13 C is the maximum estimated probe-nontarget temperature.
74.13 C is the minimum estimated probe-target temperature.
64.13 C is the optimal estimated experiment temperature.
11450 total oligo probes designed for 13492 sequences.
Job completed on Thu 19 May 2005 10:26:10 AM CDT
Total time elapsed 00h:23m:36s.