Picky parameter settings for this computation:
maximum_oligo_size = 70
minimum_oligo_size = 50
maximum_match_len = 16
minimum_match_len = 10
maximum_gc_content = 70
minimum_gc_content = 30
candidates_per_gene = 5
probes_per_gene = 1
minimum_similarity = 75
minimum_temp_separation = 10
DNA_concentration = 1
Salt_concentration = 75
single_strand_only = 0

Job started on Thu 19 May 2005 04:18:18 PM CDT
Step 1 of 6: Constructing the suffix array...
35284 target sequences.
35284 total sequences.
68639601 total DNA bases.
16380 is the longest sequence length.
Step 2 of 6: Computing invert and lcp arrays...
16380 is the longest common overlap region length among sequences.
213.78 is the average common overlap region length among sequences.
Step 3 of 6: Generating unique probe candidates...
4 CPU(s) used in this computation; step 3 is slower, be patient....
210 sequences have no unique probe due to short overlaps with others.
10645 sequences have no unique probe due to long overlaps with others.
423 sequences have no good probe candidates due to temperature limit.
99364 initial unique probe candidates detected.
Step 4 of 6: Identifying long common overlap regions...
29011 potentially sharable long common overlap regions identified.
12715 sequences hosting long common overlap regions identified.
Step 5 of 6: Generating shared probe candidates...
4 CPU(s) used in this computation; step 5 is also slow, be patient...
236 host sequences were not useful due to short overlaps.
4317 host sequences were taken by the other host sequences.
675 host sequences produce no probes due to temperature limit.
35284 additional sharable probe candidates detected.
Step 6 of 6: Determining the optimal oligo set...
2190 sequences/regions without probe at 64.37 C, 383 below, 1807 above.
2142 sequences/regions without probe at 64.59 C, 418 below, 1724 above.
2091 sequences/regions without probe at 64.89 C, 478 below, 1613 above.
2081 sequences/regions without probe at 64.93 C, 484 below, 1597 above.
2060 sequences/regions without probe at 65.07 C, 513 below, 1547 above.
2009 sequences/regions without probe at 65.28 C, 561 below, 1448 above.
1993 sequences/regions without probe at 65.63 C, 668 below, 1325 above.
2005 sequences/regions without probe at 65.83 C, 739 below, 1266 above.
1989 sequences/regions without probe at 66.07 C, 814 below, 1175 above.
2013 sequences/regions without probe at 66.38 C, 908 below, 1105 above.
2027 sequences/regions without probe at 66.67 C, 1012 below, 1015 above.
2087 sequences/regions without probe at 66.96 C, 1146 below, 941 above.
1989 sequences/regions without probe at 10.00 separation temperature.
22925 unique oligo probes designed for 22925 sequences.
4201 shared oligo probes designed for 5805 additional sequences.
16 maximum number of sequences sharing the same oligo probe.
2.39 average number of sequences sharing the same oligo probe.
61.07 C is the maximum estimated probe-nontarget temperature.
71.07 C is the minimum estimated probe-target temperature.
66.07 C is the optimal estimated experiment temperature.
27126 total oligo probes designed for 28730 sequences.
Job completed on Thu 19 May 2005 09:24:44 PM CDT
Total time elapsed 05h:06m:26s.