Picky parameter settings for this computation:
maximum_oligo_size = 70
minimum_oligo_size = 50
maximum_match_len = 17
minimum_match_len = 10
maximum_gc_content = 70
minimum_gc_content = 30
candidates_per_gene = 5
probes_per_gene = 1
minimum_similarity = 75
minimum_temp_separation = 20
DNA_concentration = 1
Salt_concentration = 75
single_strand_only = 0

Job started on Thu 19 May 2005 09:48:53 PM CDT
Step 1 of 6: Constructing the suffix array...
35284 target sequences.
35284 total sequences.
68639601 total DNA bases.
16380 is the longest sequence length.
Step 2 of 6: Computing invert and lcp arrays...
16380 is the longest common overlap region length among sequences.
213.78 is the average common overlap region length among sequences.
Step 3 of 6: Generating unique probe candidates...
4 CPU(s) used in this computation; step 3 is slower, be patient....
56 sequences have no unique probe due to short overlaps with others.
8750 sequences have no unique probe due to long overlaps with others.
1906 sequences have no good probe candidates due to temperature limit.
104110 initial unique probe candidates detected.
Step 4 of 6: Identifying long common overlap regions...
29465 potentially sharable long common overlap regions identified.
12806 sequences hosting long common overlap regions identified.
Step 5 of 6: Generating shared probe candidates...
4 CPU(s) used in this computation; step 5 is also slow, be patient...
169 host sequences were not useful due to short overlaps.
3330 host sequences were taken by the other host sequences.
1899 host sequences produce no probes due to temperature limit.
37199 additional sharable probe candidates detected.
Step 6 of 6: Determining the optimal oligo set...
7350 sequences/regions without probe at 64.44 C, 2582 below, 4768 above.
7324 sequences/regions without probe at 64.62 C, 2723 below, 4601 above.
7297 sequences/regions without probe at 64.72 C, 2808 below, 4489 above.
7269 sequences/regions without probe at 64.79 C, 2856 below, 4413 above.
7263 sequences/regions without probe at 64.82 C, 2879 below, 4384 above.
7261 sequences/regions without probe at 64.86 C, 2921 below, 4340 above.
7259 sequences/regions without probe at 64.94 C, 2995 below, 4264 above.
7268 sequences/regions without probe at 65.10 C, 3157 below, 4111 above.
7285 sequences/regions without probe at 65.25 C, 3321 below, 3964 above.
7342 sequences/regions without probe at 65.45 C, 3547 below, 3795 above.
7397 sequences/regions without probe at 65.63 C, 3749 below, 3648 above.
7259 sequences/regions without probe at 20.00 separation temperature.
20605 unique oligo probes designed for 20605 sequences.
4063 shared oligo probes designed for 5924 additional sequences.
16 maximum number of sequences sharing the same oligo probe.
2.37 average number of sequences sharing the same oligo probe.
54.94 C is the maximum estimated probe-nontarget temperature.
74.94 C is the minimum estimated probe-target temperature.
64.94 C is the optimal estimated experiment temperature.
24668 total oligo probes designed for 26529 sequences.
Job completed on Fri 20 May 2005 08:36:05 AM CDT
Total time elapsed 10h:47m:12s.