Picky parameter settings for this computation: maximum_oligo_size = 70 minimum_oligo_size = 50 maximum_match_len = 16 minimum_match_len = 10 maximum_gc_content = 70 minimum_gc_content = 30 candidates_per_gene = 5 probes_per_gene = 1 minimum_similarity = 75 minimum_temp_separation = 10 DNA_concentration = 1 Salt_concentration = 75 single_strand_only = 0 Job started on Sun 01 May 2005 06:35:39 PM CDT Step 1 of 6: Constructing the suffix array... 61251 target sequences. 61251 total sequences. 94194626 total DNA bases. 16380 is the longest sequence length. Step 2 of 6: Computing invert and lcp arrays... 9306 is the longest common overlap region length among sequences. 99.94 is the average common overlap region length among sequences. Step 3 of 6: Generating unique probe candidates... 2 CPU(s) used in this computation; step 3 is slower, be patient.... 2779 sequences have no unique probe due to short overlaps with others. 17039 sequences have no unique probe due to long overlaps with others. 1419 sequences have no good probe candidates due to temperature limit. 142086 initial unique probe candidates detected. Step 4 of 6: Identifying long common overlap regions... 52408 potentially sharable long common overlap regions identified. 19481 sequences hosting long common overlap regions identified. Step 5 of 6: Generating shared probe candidates... 2 CPU(s) used in this computation; step 5 is also slow, be patient... 1452 host sequences were not useful due to short overlaps. 11347 host sequences were taken by the other host sequences. 1051 host sequences produce no probes due to temperature limit. 22754 additional sharable probe candidates detected. Step 6 of 6: Determining the optimal oligo set... 5877 sequences/regions without probe at 64.23 C, 659 below, 5218 above. 5788 sequences/regions without probe at 64.40 C, 726 below, 5062 above. 5587 sequences/regions without probe at 64.85 C, 897 below, 4690 above. 5379 sequences/regions without probe at 65.34 C, 1093 below, 4286 above. 5329 sequences/regions without probe at 65.44 C, 1145 below, 4184 above. 5295 sequences/regions without probe at 65.52 C, 1194 below, 4101 above. 5266 sequences/regions without probe at 65.71 C, 1320 below, 3946 above. 5265 sequences/regions without probe at 65.86 C, 1424 below, 3841 above. 5240 sequences/regions without probe at 66.07 C, 1547 below, 3693 above. 5223 sequences/regions without probe at 66.27 C, 1687 below, 3536 above. 5238 sequences/regions without probe at 66.53 C, 1864 below, 3374 above. 5257 sequences/regions without probe at 66.76 C, 2039 below, 3218 above. 5236 sequences/regions without probe at 66.87 C, 2105 below, 3131 above. 5245 sequences/regions without probe at 66.93 C, 2152 below, 3093 above. 5389 sequences/regions without probe at 67.28 C, 2478 below, 2911 above. 5407 sequences/regions without probe at 67.37 C, 2557 below, 2850 above. 5571 sequences/regions without probe at 67.77 C, 2960 below, 2611 above. 5437 sequences/regions without probe at 67.45 C, 2640 below, 2797 above. 5223 sequences/regions without probe at 10.00 separation temperature. 35916 unique oligo probes designed for 35916 sequences. 3178 shared oligo probes designed for 4993 additional sequences. 13 maximum number of sequences sharing the same oligo probe. 2.38 average number of sequences sharing the same oligo probe. 61.27 C is the maximum estimated probe-nontarget temperature. 71.27 C is the minimum estimated probe-target temperature. 66.27 C is the optimal estimated experiment temperature. 39094 total oligo probes designed for 40909 sequences. Job completed on Mon 02 May 2005 04:37:18 AM CDT Total time elapsed 10h:01m:39s.