Picky parameter settings for this computation:
maximum_oligo_size = 70
minimum_oligo_size = 50
maximum_match_len = 16
minimum_match_len = 10
maximum_gc_content = 70
minimum_gc_content = 30
candidates_per_gene = 5
probes_per_gene = 1
minimum_similarity = 75
minimum_temp_separation = 10
DNA_concentration = 1
Salt_concentration = 75
single_strand_only = 0

Job started on Tue 24 May 2005 09:40:43 AM CDT
Step 1 of 6: Constructing the suffix array...
6702 target sequences.
6702 total sequences.
9081699 total DNA bases.
14733 is the longest sequence length.
Step 2 of 6: Computing invert and lcp arrays...
5580 is the longest common overlap region length among sequences.
49.39 is the average common overlap region length among sequences.
Step 3 of 6: Generating unique probe candidates...
4 CPU(s) used in this computation; step 3 is slower, be patient....
15 sequences have no unique probe due to short overlaps with others.
572 sequences have no unique probe due to long overlaps with others.
37 sequences have no good probe candidates due to temperature limit.
27677 initial unique probe candidates detected.
Step 4 of 6: Identifying long common overlap regions...
1284 potentially sharable long common overlap regions identified.
429 sequences hosting long common overlap regions identified.
Step 5 of 6: Generating shared probe candidates...
4 CPU(s) used in this computation; step 5 is also slow, be patient...
24 host sequences were not useful due to short overlaps.
77 host sequences were taken by the other host sequences.
123 host sequences produce no probes due to temperature limit.
595 additional sharable probe candidates detected.
Step 6 of 6: Determining the optimal oligo set...
66 sequences/regions without probe at 60.51 C, 7 below, 59 above.
68 sequences/regions without probe at 62.12 C, 20 below, 48 above.
78 sequences/regions without probe at 62.91 C, 37 below, 41 above.
96 sequences/regions without probe at 63.76 C, 62 below, 34 above.
81 sequences/regions without probe at 63.20 C, 43 below, 38 above.
66 sequences/regions without probe at 10.00 separation temperature.
6031 unique oligo probes designed for 6031 sequences.
123 shared oligo probes designed for 295 additional sequences.
54 maximum number of sequences sharing the same oligo probe.
4.09 average number of sequences sharing the same oligo probe.
55.47 C is the maximum estimated probe-nontarget temperature.
65.74 C is the minimum estimated probe-target temperature.
60.51 C is the optimal estimated experiment temperature.
6154 total oligo probes designed for 6326 sequences.
Job completed on Tue 24 May 2005 09:56:10 AM CDT
Total time elapsed 00h:15m:27s.