Picky parameter settings for this computation: maximum_oligo_size = 70 minimum_oligo_size = 50 maximum_match_len = 17 minimum_match_len = 10 maximum_gc_content = 70 minimum_gc_content = 30 candidates_per_gene = 5 probes_per_gene = 1 minimum_similarity = 75 minimum_temp_separation = 20 DNA_concentration = 1 Salt_concentration = 75 single_strand_only = 0 Job started on Mon 23 May 2005 10:36:24 PM CDT Step 1 of 6: Constructing the suffix array... 6702 target sequences. 6702 total sequences. 9081699 total DNA bases. 14733 is the longest sequence length. Step 2 of 6: Computing invert and lcp arrays... 5580 is the longest common overlap region length among sequences. 49.39 is the average common overlap region length among sequences. Step 3 of 6: Generating unique probe candidates... 4 CPU(s) used in this computation; step 3 is slower, be patient.... 10 sequences have no unique probe due to short overlaps with others. 540 sequences have no unique probe due to long overlaps with others. 100 sequences have no good probe candidates due to temperature limit. 27622 initial unique probe candidates detected. Step 4 of 6: Identifying long common overlap regions... 1299 potentially sharable long common overlap regions identified. 429 sequences hosting long common overlap regions identified. Step 5 of 6: Generating shared probe candidates... 4 CPU(s) used in this computation; step 5 is also slow, be patient... 17 host sequences were not useful due to short overlaps. 69 host sequences were taken by the other host sequences. 187 host sequences produce no probes due to temperature limit. 424 additional sharable probe candidates detected. Step 6 of 6: Determining the optimal oligo set... 173 sequences/regions without probe at 60.48 C, 114 below, 59 above. 161 sequences/regions without probe at 60.01 C, 92 below, 69 above. 158 sequences/regions without probe at 59.47 C, 69 below, 89 above. 158 sequences/regions without probe at 20.00 separation temperature. 5927 unique oligo probes designed for 5927 sequences. 95 shared oligo probes designed for 246 additional sequences. 54 maximum number of sequences sharing the same oligo probe. 3.86 average number of sequences sharing the same oligo probe. 49.44 C is the maximum estimated probe-nontarget temperature. 69.52 C is the minimum estimated probe-target temperature. 59.47 C is the optimal estimated experiment temperature. 6022 total oligo probes designed for 6173 sequences. Job completed on Mon 23 May 2005 10:54:14 PM CDT Total time elapsed 00h:17m:50s.