Picky parameter settings for this computation:
maximum_oligo_size = 70
minimum_oligo_size = 50
maximum_match_len = 17
minimum_match_len = 10
maximum_gc_content = 70
minimum_gc_content = 30
candidates_per_gene = 5
probes_per_gene = 1
minimum_similarity = 75
minimum_temp_separation = 10
DNA_concentration = 1
Salt_concentration = 75
single_strand_only = 0

Job started on Fri 13 May 2005 11:55:26 AM CDT
Step 1 of 6: Constructing the suffix array...
12238 target sequences.
12238 total sequences.
23015888 total DNA bases.
16380 is the longest sequence length.
Step 2 of 6: Computing invert and lcp arrays...
5749 is the longest common overlap region length among sequences.
98.21 is the average common overlap region length among sequences.
Step 3 of 6: Generating unique probe candidates...
2 CPU(s) used in this computation; step 3 is slower, be patient....
4 sequences have no unique probe due to short overlaps with others.
3441 sequences have no unique probe due to long overlaps with others.
235 sequences have no good probe candidates due to temperature limit.
38167 initial unique probe candidates detected.
Step 4 of 6: Identifying long common overlap regions...
22128 potentially sharable long common overlap regions identified.
5558 sequences hosting long common overlap regions identified.
Step 5 of 6: Generating shared probe candidates...
2 CPU(s) used in this computation; step 5 is also slow, be patient...
26 host sequences were not useful due to short overlaps.
335 host sequences were taken by the other host sequences.
933 host sequences produce no probes due to temperature limit.
20317 additional sharable probe candidates detected.
Step 6 of 6: Determining the optimal oligo set...
763 sequences/regions without probe at 63.24 C, 250 below, 513 above.
777 sequences/regions without probe at 64.05 C, 365 below, 412 above.
835 sequences/regions without probe at 64.93 C, 515 below, 320 above.
778 sequences/regions without probe at 64.07 C, 370 below, 408 above.
763 sequences/regions without probe at 10.00 separation temperature.
8386 unique oligo probes designed for 8386 sequences.
2363 shared oligo probes designed for 3659 additional sequences.
52 maximum number of sequences sharing the same oligo probe.
2.43 average number of sequences sharing the same oligo probe.
58.22 C is the maximum estimated probe-nontarget temperature.
68.31 C is the minimum estimated probe-target temperature.
63.24 C is the optimal estimated experiment temperature.
10749 total oligo probes designed for 12045 sequences.
Job completed on Fri 13 May 2005 02:57:58 PM CDT
Total time elapsed 03h:02m:32s.