Loading and analyzing oligo file unscreened_HBV_shRNA.seq...
  ==> Use the better nonlinear salt effect equation

Picky parameter settings used for this examination:
maximum_oligo_size = 21
minimum_oligo_size = 21
minimum_match_len = 10
minimum_temp_separation = 0
DNA_concentration = 1
Salt_concentration = 500
single_strand_only = 1

Job started on Tue 06 Oct 2009 07:28:48 PM CDT
Step 1 of 3: Reading input probe sequences...
31 input probe sequences identified.
683 total DNA bases and probe end markers read.
Step 2 of 3: Examining probes on target sequences...
2 CPU(s) used in this computation step.
0 probes have no identifiable targets in the gene sets.
0 probes violate target/nontarget temperature separation limit.
Step 3 of 3: Analyzing probe characteristics...
21 is the longest oligo probe length.
21 is the shortest oligo probe length.
29 unique probes targeting 1 sequences
2 shared probes targeting 0 additional sequences.
2 maximum number of sequences sharing the same oligo probe.
2.00 average number of sequences sharing the same oligo probe.
69.49 C is the maximum estimated probe-nontarget temperature.
56.23 C is the minimum estimated probe-target temperature.
-13.26 C is the closest estimated temperature separation.
57.45 C is the medium estimated probe-nontarget temperature.
69.53 C is the medium estimated probe-target temperature.
12.08 C is the temperature separation between the mediums.
31 total useful oligo probes found to target 1 sequences.
Job completed on Tue 06 Oct 2009 07:28:48 PM CDT
Total time elapsed 00h:00m:00s.