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Data Source
For the Mouse sequences, They are mRNAs, with their accession format either NM_xxxxxx (curated RefSeq) or XM_xxxxxx (model prediction). Certain extra long sequences were broken into pieces to produce the chopped sequences for execution.
Common Design Parameters
Most design parameters of Picky depends on the experimental conditions. The following design parameters are used to generate the collection of oligos in this page. Except for the maximum_match_len and the minimum_temp_separation parameters, all the other parameters are fixed and their values are shown:
| maximum_oligo_size = 70 |
minimum_oligo_size = 50 |
| maximum_match_length = 15 |
minimum_match_length = 10 |
| maximum_gc_content = 70 |
minimum_gc_content = 30 |
| candidates_per_gene = 5 |
probes_per_gene = 1 |
| minimum_similarity = 75 |
minimum_temp_separation = 20 |
| DNA_concentration = 1 |
Salt_concentration = 75 |
Variable Design Parameters
Oligo sets can be designed with different strigency by varying the maximum match length and minimum temperature separation parameters of Picky. Generally, a bigger oligo set can be produced when the maximum match is increased, or when the minimum temperature is descreased. However, making those changes also decreased the discriminative power of the oligo designed. Therefore, it is up to the users to decide which oligo set gives them the best balance between number of oligos and the discriminative power. A picture is provided below to illustrate the difference:
Download Oligo Sets
Total Sequences 25392 |
Maxinum Match Length |
| Temperature Seperation
|
15 |
16 |
20°C |
(11039 oligos, 4225sec) |
(18747 oligos, 57145 sec) |
10°C |
(16487 oligos, 4276 sec) |
(21463 oligos, 56292 sec) |
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