Loading target files

Picky accepts any sequence of genome as a text file. Simply left click the Open target files or select Files > Load target genes from the pull down menu. In this tutorial, we will use the maize_est_contig.seq, a sequence coming from maize corn. Select the file and choose open. A pop up box will display:

It is always best to input complete base sequences, however, the user may choose whether they want Picky to randomly assign unknown sequences or if they want these unknown bases to remain in the data set. For the purposes of this tutorial, choose Yes.

The sequence is now loaded into the program. Use the scroll down menus to view the sequences and numbering.

Entering Oligo Design Parameters

To specify and compute oligo probes left click the Compute Oligo or choose Oligo > Compute from the pull down menu. The following screen appears: